Member Labs

  Name Room Phone Email Websites
  Dr. Derek J. Wilson 331C LSB x20786
  Dr. Demian Ifa

252 CB

  Dr. Deithard Bohme

260 CB

  Dr. K.W. Michael Siu 242 CB x58426
  Dr. Alan C. Hopkinson 248 CB

  Dr. John C. McDermott 427B LSB x30344
  Dr. Sergey Krylov 340 PSE x22345

Derek Wilson

Dr. Wilson

Dr. Wilson received his doctorate from the University of Western Ontario in 2006. His doctoral work under Dr. Lars Konermann centered on the introduction of new Mass Spectrometry and Microfluidics-based technologies for the investigation of rapid dynamic processes in proteins. Dr. Wilson held the top level National Science and Engineering Research Council (NSERC) award, the Canada Graduate Scholarship, throughout his graduate career. He then took up an NSERC post-doctoral fellowship for tenure in the Dobson group at Cambridge . His post-doctoral work on amyloidogenic proteins, including Transtherytin and S. Solfataricus Acylphosphatase extended his expertise to biological NMR, with a focus on biophysical methods. Dr. Wilson's work is currently focused on bioanalytical chemistry, particularly on the relationship between protein structure, dynamics and function. Highlights of his work include structural characterization of an 'uncharacterizable' Alzheimer's-associated protein, charcterization of drug binding to the cancer-associated protein STAT3 and introduction of a new model for how conformational dynamics is linked to catalysis in enzymes. Dr. Wilson has published articles in top ranked international journals including Nature Leukemia, Angewandte Chemie, Analytical Chemistry, Biochemistry and the Biophysical Journal. He has also co-authored three book chapters in Protein Structures (Nova Science), Comprehensive Analytical Chemistry (Elsevier) and The Encyclopedia of Mass Spectrometry (Elsevier). His technology for measuring rapid solution phase dynamic processes by Mass Spectrometry was awarded a U.S. Patent in 2005 (#6,870,154). He has attracted substantial funding from NSERC, the Alzheimer's Society of Canada and the Ontario Ministry of Research and Innovation.

Derek Wilson

Dr. Ifa

Dr. Ifa's research focuses on the development and application of mass spectrometry to bioanalysis. Major areas of interest are: (i) mass spectrometry imaging for the determination of the spatial distributions of chemical constituents of a tissue or other samples, (ii) chemical cross-linking for low-resolution characterization of the conformation, assembly, and interactions of macromolecules in solution, and (iii) discovery and validation of biomarkers for diagnosis and progression of disorders of clinical relevance.

Derek Wilson

Dr. Bohme

Dr. Bohme's research  has focused on the investigation of metallic, organometallic and biometallic ion chemistry as well as biological ions and their chemistry. An inductively coupled plasma / selected-ion flow tube / triple quadrupole (ICP/SIFT/QqQ) mass spectrometer has been used to study bare metal-ion chemistry and two electrospray ionization (ESI)/triple quadrupole mass spectrometers are providing information on the breakdown of selected organometallic, biological and biometallic ions. Also, an ESI/q/SIFT/QqQ configuration has been developed to study reactions of small molecules with biological and biometallic ions, including positive and negative ions of penicillins, siderophores, porphorins, phthalocyanines and oligonucleotides.

Derek Wilson

Dr. Siu

Dr. Siu’s group investigates the chemistries underlying reactions and processes that occur in mass spectrometry (MS), develops new ionization and fragmentation techniques and methods for MS, and applies these chemistries, techniques and methods for the analysis of real-world samples. The fragmentation of protonated peptides constitutes the basis for protein identification in proteomics either de novo or via a library search.  Despite this wide adaptation, the chemistries involved are still poorly understood and, at present, only a fraction of the MS/MS spectra yields positive peptide identification.  The Siu group continues to examine some of the poorly understood fragmentation chemistries, in particular those that are mediated by cyclized intermediates, which could lead to a loss of the original amino acid sequence information.  Peptides can also be ionized (and made amenable to MS analysis) via one-electron oxidation to give their molecular radical cations.  Under appropriate conditions, this reaction can be mediated by a metal centre, typically Cu2+.  Other metal centres are currently being investigated and some have been found to show exceptional promises.  Peptide radical ions, in general, exhibit richer fragmentation chemistries than their protonated analogues because dissociation can be induced by the charge as well as the radical. The examinations of structures and chemistries are carried out in collaboration with Professor Alan Hopkinson.

Dr.Siu’s group applies mass spectrometry to proteomic analyses in collaboration with cancer pathologists and surgeons.  Biomarkers for endometrial, head-and-neck, renal and brain cancers have been identified and verified.  Some head-and-neck cancer biomarkers are currently in use in a prognostic test for oral maxillofacial surgery clinics to identify lesions that have a high probability of cancer transformation.

Derek Wilson

Dr. Hopkinson

Dr. Hopkinson's research is in the area of application of computational chemistry to the calculation of ion structures and energetics. The bulk of his research has been carried out in collaboration with Professors Bohme and Siu. These calculations are crucial to mass spectrometry research as they provide plausible structures and energetics of experimentally measured ions and transition states involved in fragmentation pathways. These allow fragmentation mechanisms to be proposed and ion chemistry rationalized. Ions that have been examined range from cyclic a2 ions, dipositive and tripositive metal-centred ions, protonated and metalated oligopeptides, polysodiated ions, protonated a3 ions, and peptide radical cations."

Derek Wilson

Dr. McDermott

Dr. McDermott's mass spectrometry-related research centers on the phosphorylation status of an important protein, myocyte enhancer factor (MEF) 2A, which is a member of a family of transcriptional regulators recognized for heart and skeletal muscle development and in the protection of neuronal cells from apotosis. In the last year, several novel phosphorylation sites on MEF2A were identified, and significant advancement in the understanding of MEF2A regulation was also made. These constitute the basis of a collaboration with Stratagene, La Jolla, CA, on the development of tandem affinity purification for producing significant quantities of proteins for mass spectrometry characterization.

Derek Wilson

Dr. Krylov

Dr. Krylov is a Canada Research Chair in Bioanalytical Chemistry. He pioneered novel tools for study of biomolecular interactions in vitro and in live cells, termed Kinetic Capillary Electrophoresis (KCE) and Chemical Cytometry. KCE is dubbed an “Analytical Swiss Army knife”, and can be used for studying kinetics and thermodynamics of biomolecular interactions, for generation of “smart” affinity ligands from complex mixtures, as well as for application of such “smart” ligands for quantitative analysis of biomolecules. Chemical Cytometry is a novel bioanalytical tool which facilitates studies of biomolecular interactions at the single-cell level. Dr. Krylov has recently extended his research program to a new direction: Aptamer-facilitated Biomarker Discovery (AptaBiD). The AptaBiD technology uniquely allows for discovery of biomarkers in their native states on the cell surface.